Clinically quantifiable measures from an in vitro study of nucleus augmentation of the intervertebral disc

Abstract

Purpose: Nucleus augmentation has been proposed as an early-stage intervention for intervertebral disc degeneration and involves the injection of a biomaterial into the nucleus to restore disc height and functionality.  The aim of this work was to identify clinically relevant quantitative measures that indicate the mechanical performance of the disc following nucleus augmentation.

Method: Bovine tail bone-disc-bone units (n=22) were mechanically tested under cyclic loading sequentially in native, artificially degenerated and treated states. Treatment involved injection of a peptide:glycosaminoglycan mixture into the degenerated disc to a predetermined load using a syringe driver with an integrated force sensor. The mechanical restoration of the treatment was determined by comparing the biomechanical behaviour of the native state to the treated state of each disc. The mechanical restoration was then compared against clinically quantitative parameters.

Results: Significant biomechanical differences were observed from the degenerate state to the native and treated states. The force delivered during injection was found to ramp to a steady state, followed by a final rapid increase, however all measures associated with injection force poorly correlated with level of mechanical restoration. Direct measures of the disc, volume injected and change in disc height from injection, had the strongest relationship to mechanical restoration.

Conclusion: This work showed that measuring the injection force for injectable treatments of the disc can provide lower and upper limits for delivery, but direct measures are stronger indicators of disc mechanical restoration.